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基因资源挖掘与利用
 

组长：王克剑

电话：0571-63370202

邮箱：wangkejian@caas.cn

所属部门：水稻生物学重点实验室

 

 
 

王克剑，研究员，博士生导师，1983年12月生，江苏盐城人；现为中国水稻研究所研究员、博士生导师、水稻生物学国家重点实验室副主任、中国农业科学院科技创新工程“水稻染色体工程及基因组编辑”创新团队首席科学家，农业农村部“杰出青年农业科学家”、中国农科院“青年英才”。

2004年于扬州大学获农学学士学位，2009年于中国科学院遗传与发育生物学研究所获理学博士学位并留所工作，先后任助理研究员、副研究员。2013年8月至中国农业科学院/中国水稻研究所任课题组长，主要围绕有性生殖和基因组编辑等进行理论研究和技术研发工作。先后发表论文40余篇，其中以通讯作者和第一作者在Nature Biotechnology、Molecular Plant和Plant Biotechnology Journal等期刊上发表论文20余篇。现任中国作物学会第十一届理事会理事、中国遗传学会基因组编辑分会委员；担任BMC Plant Biology、aBIOTECH和作物学报等期刊编委。

——近年发表的主要论文

（*通讯作者，#共同一作）

  1. Xu Y#., Meng X#., Wang J, Qin B, Wang K., Li J, Wang C*, Yu H*. (2020) ScCas9 recognizes NNG protospacer adjacent motif in genome editing of rice. Science China Life Sciences doi.org/10.1007/s11427-019-1630-2

  2. Wang C., Liu Q., Shen Y., Hua Y., Wang J., Lin J., Wu M., Sun T., Cheng Z., Mercier R., Wang K*. (2019) Clonal seeds from hybrid rice by simultaneous genome engineering of meiosis and fertilization genes. Nature Biotechnology, 37(3):283-287. (封面文章，CCTV新闻：http://news.cctv.com/2019/01/11/ARTIFb1TVwjp8t7fgrQp3ti0190111.shtml )

  3. Wang K. (2019). Fixation of hybrid vigor in rice: synthetic apomixis generated by genome editing. aBIOTECH 1, 15-20.

  4. Wang C., Wang K*. (2019) Rapid Screening of CRISPR/Cas9-Induced Mutants Using the ACT-PCR Method. vol 1917. Humana Press, New York, NY.

  5. Wang J#., Wang C#., Wang K*. (2019) Generation of marker-free transgenic rice using CRISPR/Cas9 system controlled by floral specific promoters. Journal of Genetics and Genomics, 46(1): 61-64.

  6. Ren J#., Hu X#., Wang K., Wang C. (2019) Development and application of CRISPR/Cas system in rice. Rice Science, 26(2):69-76.

  7. Hu X#., Meng X#., Li J., Wang K*., and Yu H*. (2019) Improving the efficiency of the CRISPR-Cas12a system with tRNA-crRNA arrays. The Crop Journal. https://doi.org/10.1016/j.cj.2019.06.007

  8. Wang J#., Meng X#., Hu X#., Sun T., Li J., Wang K*., Yu H*. (2019) xCas9 expands the scope of genome editing with reduced efficiency in rice. Plant Biotechnology Journal, 17(4):709-711.

  9. Liu Q#., Wang C#., Jiao X., Zhang H., Song L., Li Y., Gao C., and Wang K*. (2019) Hi-TOM: a platform for high-throughput tracking of mutations induced by CRISPR/Cas systems. Science China Life Sciences, 62(1):1-7. (封面文章)

  10. Li S#., Shen L#., Hu P., Liu Q., Zhu X., Qian Q., Wang K*., and Wang Y*. (2019) Developing disease-resistant thermosensitive male sterile rice by multiplex gene editing. Journal of Integrative Plant Biology, 61(12), 1201-1205.

  11. Hu, X#., Meng, X#., Liu, Q., Li, J*., and Wang K*. (2018) Increasing the efficiency of CRISPR-Cas9-VQR precise genome editing in rice. Plant Biotechnology Journal, 16: 292-297. (其中第一作者胡熙璕为农业推广硕士)

  12. Meng, X#., Hu, X#., Liu, Q., Song, X., Gao, C., Li, J*., and Wang, K*. (2018) Robust genome editing of CRISPR-Cas9 at NAG PAMs in rice. Science China Life Sciences, v.61(01):124-127. (其中第一作者胡熙璕为农业推广硕士)

  13. Shen, L#., Wang, C#., Fu, Y., Wang, J., Liu, Q., Zhang, X., Yan, C*., Qian, Q*., and Wang, K*. (2018) QTL editing confers opposing yield performance in different rice varieties. Journal of Integrative Plant Biology, 61: 122-125. (封面文章、年度最佳论文)

  14. Zhan N., Wang C., Chen L., Yang H., Feng J., Gong X., Ren B., Wu R., Mu J., Li Y., Liu Z., Zhou Y., Peng J., Wang K., Huang X., Xiao S., Zuo J. (2018) S-Nitrosy lation targets GSNO reductase for selective autophagy during hypoxia responses in plants. Molecular Cell, 71(1):142-154.

  15. Hua, Y#., Wang, C#., Huang, J#., and Wang, K*. (2017) A simple and efficient method for CRISPR/Cas9 mutant screening. Journal of Genetics and Genomics, 44:213.

  16. Hu, X#., Wang, C#., Liu, Q., Fu, Y., and Wang, K*. (2017) Targeted mutagenesis in rice using CRISPR-Cpf1 system. Journal of Genetics and Genomics, 44:71-73. (其中第一作者胡熙璕为农业推广硕士)

  17. Shen, L#., Hua, Y#., Fu, Y#., Li, J#., Liu, Q., Jiao, X., Xin, G., Wang, J., Wang, X., Yan, C*., and Wang, K*. (2017) Rapid generation of genetic diversity by multiplex CRISPR/Cas9 genome editing in rice. Science China Life Sciences, 5:506-515. (基因编辑专刊)

  18. Zhang P., Zhang Y., Sun L., Sinumporn S., Yang Z., Sun B., Xuan D., Li Z., Yu P., Wu W., Wang K., Cao L., Cheng S. (2017) The rice AAA-ATPase OsFIGNL1 is essential for male meiosis. Frontiers in Plant Science, 8:0-1639.

  19. Hu, X#., Wang, C#., Fu, Y#., Liu, Q., Jiao, X., and Wang, K*. (2016) Expanding the range of CRISPR/Cas9 genome editing in rice. Molecular Plant, 9:943-945. (其中第一作者胡熙璕为农业推广硕士)

  20. Wang, K*., Wang, C., Liu, Q., Fu, Y. (2015) Increasing the genetic recombination frequency by partial loss of function of the synaptonemal complex in rice. Molecular Plant, 8:1295-1298.

  21. Wang, C#., Shen, L#., Fu, Y., Yan, C., Wang, K*. (2015) A simple CRISPR/Cas9 system for multiplex genome editing in rice. Journal of Genetics and Genomics, 42:703-706.

  22. Che, L#., Wang, K#., Tang, D., Liu, Q., Chen, X., Li, Y., Hu, Q., Shen, Y., Yu, H., Gu, M., et al. (2014) OsHUS1 facilitates accurate meiotic recombination in rice. PLoS Genetics, 10:e1004405.

  23. Wu, X#., Tang, D#., Li, M#., Wang, K., Cheng, Z*. (2013) Loose plant architecture 1, an INDETERMINATE domain protein involved in shoot gravitropism, regulates plant architecture in rice. Plant Physiology, 161:317-329.

  24. Ji, J#., Tang D#., Wang, M., Li, Y., Zhang L, Wang, K., Li, M., and Cheng, Z*. (2013) MRE11 is required for homologous synapsis and DSB processing in rice meiosis. Chromosoma, 122:363-376.

  25. Luo, Q#., Tang, D#., Wang, M., Luo, W., Zhang, L., Qin, B., Shen, Y., Wang, K., Li, Y., Cheng, Z*. (2013) The role of OsMSH5 in crossover formation during rice meiosis. Molecular Plant, 6:729-742.

  26. Wang, K#., Wang, M#., Tang, D#., Shen, Y., Miao, C., Hu, Q., Lu, T., Cheng, Z*. (2012) The role of HEI10 in crossover formation in rice. PLoS Genetics, 8:e1002809.

  27. Ji, J#., Tang, D#., Wang, K., Wang, M., Che, L., Li, M., Cheng, Z*. (2012) The role of OsCOM1 in homologous chromosome synapsis and recombination in rice meiosis. The Plant Journal, 72:18-30.

  28. Hong, L#., Tang, D#., Zhu, K#., Wang, K., Li, M., and Cheng, Z*. (2012) Somatic and reproductive cell development in rice anther is regulated by a putative glutaredoxin. The Plant Cell, 24:577-588.

  29. Wang, M#., Tang, D#., Luo, Q., Jin, Y., Shen, Y., Wang, K., Cheng, Z*. (2012) BRK1, a Bub1-related kinase, is essential for generating proper tension between homologous kinetochores at metaphase I of rice meiosis. The Plant Cell, 24:4961-4973.

  30. Shen, Y#., Tang, D#., Wang, K., Wang, M., Huang, J., Luo, W., Luo, Q., Hong, L., Li, M., Cheng, Z*. (2012) The role of ZIP4 in homologous chromosome synapsis and crossover formation in rice meiosis. Journal of Cell Science, 125:2581-2591.

  31. Hong, L#., Tang, D#., Shen, Y., Hu, Q., Wang, K., Li, M., Lu, T., and Cheng, Z*. (2012) MIL2 (MICROSPORELESS2) regulates early cell differentiation in the rice anther. New Phytologist, 196:402-413.

  32. Hong, L., Qian, Q., Tang, D., Wang, K., Li, M., and Cheng, Z*. (2012) A mutation in the rice chalcone isomerase gene causes the golden hull and internode 1 phenotype. Planta, 236:141-151.

  33. Wang, K#., Wang, M#., Tang, D#., Shen, Y., Qin, B., Li, M., and Cheng, Z*. (2011) PAIR3, an axis-associated protein, is essential for the recruitment of recombination elements onto meiotic chromosomes in rice. Molecular Biology of the Cell, 22:12-19.

  34. Che, L#., Tang, D#., Wang, K., Wang, M., Zhu, K., Yu, H., Gu, M., and Cheng, Z*. (2011) OsAM1 is required for leptotene-zygotene transition in rice. Cell Research, 21:654-665.

  35. Shao, T#., Tang, D#., Wang, K., Wang, M., Che, L., Qin, B., Yu, H., Li, M., Gu, M., and Cheng, Z*. (2011) OsREC8 is essential for chromatid cohesion and metaphase I monopolar orientation in rice meiosis. Plant Physiology, 156:1386-1396.

  36. Wang, M., Tang, D., Wang, K., Shen, Y., Qin, B., Miao, C., Li, M., and Cheng, Z*. (2011) OsSGO1 maintains synaptonemal complex stabilization in addition to protecting centromeric cohesion during rice meiosis. The Plant Journal, 67:583-594.

  37. Li, M., Tang, D., Wang, K., Wu, X., Lu, L., Yu, H., Gu, M., Yan, C*., and Cheng, Z*. (2011) Mutations in the F-box gene LARGER PANICLE improve the panicle architecture and enhance the grain yield in rice. Plant Biotechnology Journal, 9:1002-1013.

  38. Qin, B., Tang, D., Huang, J., Li, M., Wu, X., Lu, L., Wang, K., Yu, H., Chen, J., Gu, M., and Cheng, Z*. (2011) Rice OsGL1-1 is involved in leaf cuticular wax and cuticle membrane. Molecular Plant, 4:985-995.

  39. Wang, K#., Tang, D#., Hong, L., Xu, W., Huang, J., Li, M., Gu, M., Xue, Y*., Cheng, Z*. (2010) DEP and AFO regulate reproductive habit in rice. PLoS Genetics, 6:e1000818. (封面文章)

  40. Wang, M., Wang, K., Tang, D., Wei, C., Li, M., Shen, Y., Chi, Z., Gu, M., Cheng, Z*. (2010) The central element protein ZEP1 of the synaptonemal complex regulates the number of crossovers during meiosis in rice. The Plant Cell, 22:417-430.

  41. Yu, H#., Wang, M#., Tang, D., Wang, K., Chen, F., Gong, Z., Gu, M.*, and Cheng, Z*. (2010) OsSPO11-1 is essential for both homologous chromosome pairing and crossover formation in rice. Chromosoma, 119:625-636.

  42. Wang, K., Tang, D., Wang, M., Lu J., Yu, H., Liu, J., Qian, B., Gong, Z., Wang, X., Chen, J., Gu, M., and Cheng, Z*. (2009) MER3 is required for normal meiotic crossover formation, but not for presynaptic alignment in rice. Journal of Cell Science, 122:2055-2063.

课题组成员

研究与技术人员：王克剑、王春、刘庆、刘朝雷、焦晓真

 

联 系 人：王克剑

联系电话：0571-63370202 传真：0571-63370202 电子邮箱：wangkejian@caas.cn

 
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内容更新日期：2020-03-13