Further insight into the role of KAN1, a member of KANADI transcription factor family in rice
Yawo Mawunyo Nevame Adedze, Baobing Feng, Lei Shi, Zhonghua Sheng, Shaoqing Tang, Xiangjin Wei* and Peisong Hu*
Plant Growth Regulation
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10.1007/s10725-017-0335-7
Abstract
The rice EMS-derived mutant leaf adaxialized1 (lad1) was isolated based on its upward rolling leaf phenotype.Besides the adaxially rolled leaf, many other agronomictraits were also compromised in lad1. The rollingtrait was characterized by a noticeable alteration of bulliformcells in the adaxial side of the leaves. Map-based cloningshowed a single nucleotide substitution in the promoterregion of the KAN1 gene in lad1 mutant. Further, overexpressingand CRISPR/cas9-edited knockdown transgenicplants confirmed that KAN1 was responsible for the mutantphenotype of lad1. Yeast two-hybrid and bimolecular fluorescencecomplementation assay demonstrated that KAN1 can interact with the auxin response factors ARF3, ARF7and ARF15. Physiologically, the contents of auxin (IAA),abscisic acid (ABA), jasmonic acid (JA) and gibberellin(GA) were all significantly increased in the lad1 mutant.Moreover, the GA3 content dramatically decrease in wildtype,but increased in lad1 under IAA induction. Additionally,the expression levels of several IAA and GA biosynthesisand responsive-related genes and genes involved inleaf polarity determination were altered in lad1. Therefore,we hypothesized that KAN1/ARFs protein complexes act as auxin-dependent regulatory units that play a conserved rolein leaf development.