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A Nck-associated protein 1-like protein affects drought sensitivity by its involvement in leaf epidermal development and stomatal closure in rice
Lichao Huang+, Long Chen+, Lan Wang, Yaolong Yang, Yuchun Rao, Deyong Ren, Liping Dai, Yihong Gao, Weiwei Zou, Xueli Lu, Guangheng Zhang, Li Zhu, Jiang Hu, Guang Chen, Lan Shen, Guojun Dong, Zhenyu Gao, Longbiao Guo, Qian Qian* and Dali Zeng*
Plant Journal , 2019,98(5):884-897
DOI:10.1111/tpj.14288

Abstract


Water deficit is a major environmental threat affecting crop yields worldwide. In this study, a drought stress‐sensitive mutant drought sensitive 8 (ds8) was identified in rice (Oryza sativa L.). The DS8 gene was cloned using a map‐based approach. Further analysis revealed that DS8 encoded a Nck‐associated protein 1 (NAP1)‐like protein, a component of the SCAR/WAVE complex, which played a vital role in actin filament nucleation activity. The mutant exhibited changes in leaf cuticle development. Functional analysis revealed that the mutation of DS8 increased stomatal density and impaired stomatal closure activity. The distorted actin filaments in the mutant led to a defect in abscisic acid (ABA)‐mediated stomatal closure and increased ABA accumulation. All these resulted in excessive water loss in ds8 leaves. Notably, antisense transgenic lines also exhibited increased drought sensitivity, along with impaired stomatal closure and elevated ABA levels. These findings suggest that DS8 affects drought sensitivity by influencing actin filament activity.